spatial transcriptomics cell-type deconvolution Search Results


αtc1 6 cells  (ATCC)
95
ATCC αtc1 6 cells
αtc1 6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hy 110203 type ii collagenase worthington biochemical  (Worthington Biochemical)
99
Worthington Biochemical hy 110203 type ii collagenase worthington biochemical
Hy 110203 Type Ii Collagenase Worthington Biochemical, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcriptase  (Thermo Fisher)
90
Thermo Fisher reverse transcriptase
Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine macrophage cell line raw 264 7  (ATCC)
99
ATCC murine macrophage cell line raw 264 7
Murine Macrophage Cell Line Raw 264 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines hl60 female atcc atcc ccl  (ATCC)
99
ATCC cell lines hl60 female atcc atcc ccl
Cell Lines Hl60 Female Atcc Atcc Ccl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek 293t atcc  (ATCC)
99
ATCC hek 293t atcc
Hek 293t Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hct116 atcc  (ATCC)
99
ATCC hct116 atcc
Hct116 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htert immortalized adipose  (ATCC)
96
ATCC htert immortalized adipose
Htert Immortalized Adipose, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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htert immortalized adipose - by Bioz Stars, 2026-03
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8065 met 5a cells atcc cat  (ATCC)
96
ATCC 8065 met 5a cells atcc cat
8065 Met 5a Cells Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2638 h22 cells  (ATCC)
99
ATCC 2638 h22 cells
2638 H22 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2638 h22 cells/product/ATCC
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4370s phospho nf kb p65 ser536  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 4370s phospho nf kb p65 ser536
Figure 6. Mct4 activates <t>p65-IL-10</t> axis to promote Mtb survival by translocating intracellular lactate out of macrophages (A) Intracellular and extracellular lactate level was detected in Slc16a3+/+ and Slc16a3/ BMDMs at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (B) Intracellular and extracellular lactate level was detected in Raw 264.7 cells overexpressing Mct4 at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (C) Phosphorylation level of <t>NF-kB</t> p65 was detected by western blot in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 60 min. The ratio of expression of phosphorylation of NF-kB p65 and NF-kB p65 is shown in graph. b-Actin served as an internal control. (D) Intracellular mRNA and production of IL-10 were detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 1 for 48 h by qRT-PCR and ELISA analysis. b-Actin served as an internal control. (E) The intracellular Mtb load was detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 48 h by CFU assay. (A–E) Western blot results were representative of three independent experiments with similar results. Data shown were the mean G SD and are from at least three independent experiments. Data were analyzed by unpaired t test and two-way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.
4370s Phospho Nf Kb P65 Ser536, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4370s phospho nf kb p65 ser536/product/Cell Signaling Technology Inc
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thp 1 cells  (ATCC)
99
ATCC thp 1 cells
Figure 6. Mct4 activates <t>p65-IL-10</t> axis to promote Mtb survival by translocating intracellular lactate out of macrophages (A) Intracellular and extracellular lactate level was detected in Slc16a3+/+ and Slc16a3/ BMDMs at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (B) Intracellular and extracellular lactate level was detected in Raw 264.7 cells overexpressing Mct4 at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (C) Phosphorylation level of <t>NF-kB</t> p65 was detected by western blot in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 60 min. The ratio of expression of phosphorylation of NF-kB p65 and NF-kB p65 is shown in graph. b-Actin served as an internal control. (D) Intracellular mRNA and production of IL-10 were detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 1 for 48 h by qRT-PCR and ELISA analysis. b-Actin served as an internal control. (E) The intracellular Mtb load was detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 48 h by CFU assay. (A–E) Western blot results were representative of three independent experiments with similar results. Data shown were the mean G SD and are from at least three independent experiments. Data were analyzed by unpaired t test and two-way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.
Thp 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thp 1 cells/product/ATCC
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Image Search Results


Figure 6. Mct4 activates p65-IL-10 axis to promote Mtb survival by translocating intracellular lactate out of macrophages (A) Intracellular and extracellular lactate level was detected in Slc16a3+/+ and Slc16a3/ BMDMs at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (B) Intracellular and extracellular lactate level was detected in Raw 264.7 cells overexpressing Mct4 at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (C) Phosphorylation level of NF-kB p65 was detected by western blot in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 60 min. The ratio of expression of phosphorylation of NF-kB p65 and NF-kB p65 is shown in graph. b-Actin served as an internal control. (D) Intracellular mRNA and production of IL-10 were detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 1 for 48 h by qRT-PCR and ELISA analysis. b-Actin served as an internal control. (E) The intracellular Mtb load was detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 48 h by CFU assay. (A–E) Western blot results were representative of three independent experiments with similar results. Data shown were the mean G SD and are from at least three independent experiments. Data were analyzed by unpaired t test and two-way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.

Journal: iScience

Article Title: Monocarboxylate transporter 4 facilitates Mycobacterium tuberculosis survival through NF-κB p65-mediated interleukin-10 production.

doi: 10.1016/j.isci.2024.110238

Figure Lengend Snippet: Figure 6. Mct4 activates p65-IL-10 axis to promote Mtb survival by translocating intracellular lactate out of macrophages (A) Intracellular and extracellular lactate level was detected in Slc16a3+/+ and Slc16a3/ BMDMs at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (B) Intracellular and extracellular lactate level was detected in Raw 264.7 cells overexpressing Mct4 at 0, 24, and 48 h of Mtb infection at MOI of 1 by lactate assay kit. (C) Phosphorylation level of NF-kB p65 was detected by western blot in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 60 min. The ratio of expression of phosphorylation of NF-kB p65 and NF-kB p65 is shown in graph. b-Actin served as an internal control. (D) Intracellular mRNA and production of IL-10 were detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 1 for 48 h by qRT-PCR and ELISA analysis. b-Actin served as an internal control. (E) The intracellular Mtb load was detected in Raw 264.7 cells overexpressing Mct4 pretreated with 25 mM lactate for 3 h upon Mtb infection at MOI of 5 for 48 h by CFU assay. (A–E) Western blot results were representative of three independent experiments with similar results. Data shown were the mean G SD and are from at least three independent experiments. Data were analyzed by unpaired t test and two-way ANOVA, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.

Article Snippet: eFluor 450-CD80 (16-10A1) Invitrogen Cat #48-0801-82 Super Bright 600-CD86 (GL6) eBioscience Cat #63-0862-82 PE-MHC II (M5/114.15.2) TONBO Cat #50-5321-U100 IL1b Rabbit pAb ABclonal Cat #A16288 NF-kB p65 (D14E12) Rabbit mAb Cell Signaling Technology Cat #8242S p38 MAPK (D13E1) Rabbit mAb Cell Signaling Technology Cat #8690S Phospho-p38 MAPK (Thr180/Tyr182) Rabbit mAb Cell Signaling Technology Cat #4511S Stat3 (D3Z2G) Rabbit mAb Cell Signaling Technology Cat #12640 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb Cell Signaling Technology Cat #9145 Stat1 (D1K9Y) Rabbit mAb Cell Signaling Technology Cat #14994 Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb Cell Signaling Technology Cat #8826S Erk1/2 Rabbit mAb Cell Signaling Technology Cat #4695S Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mAb Cell Signaling Technology Cat #4370S Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat #3033S F4/80 Monoclonal Antibody (BM8) eBioscience Cat #14-4801-82 Phospho-NF-kB p65 (Ser536) Antibody Affinity Cat #AF2006 IL-10 neutralizing antibody eBioscience Cat #16-7102-81 Bacterial and virus strains Mtb strain of H37Rv American Type Culture Collection NO.7294 Chemicals, peptides, and recombinant proteins SB203580 Selleckchem Cat #S1076 JSH23 Selleckchem Cat #S7351 Lactate Selleckchem Cat #E4473 Experimental models: cell lines Raw 264.7 American Type Culture Collection, USA N/A

Techniques: Infection, Lactate Assay, Phospho-proteomics, Western Blot, Expressing, Control, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Colony-forming Unit Assay

Figure 7. Potential mechanism for Mct4 to promote Mtb survival in macrophages Mtb infection increases Mct4 expression in macrophages, and Mct4 transports lactate outside of macrophages to decrease intracellular lactate, which promotes NF-kB p65 activation and IL-10 production, eventually facilitating Mtb survival.

Journal: iScience

Article Title: Monocarboxylate transporter 4 facilitates Mycobacterium tuberculosis survival through NF-κB p65-mediated interleukin-10 production.

doi: 10.1016/j.isci.2024.110238

Figure Lengend Snippet: Figure 7. Potential mechanism for Mct4 to promote Mtb survival in macrophages Mtb infection increases Mct4 expression in macrophages, and Mct4 transports lactate outside of macrophages to decrease intracellular lactate, which promotes NF-kB p65 activation and IL-10 production, eventually facilitating Mtb survival.

Article Snippet: eFluor 450-CD80 (16-10A1) Invitrogen Cat #48-0801-82 Super Bright 600-CD86 (GL6) eBioscience Cat #63-0862-82 PE-MHC II (M5/114.15.2) TONBO Cat #50-5321-U100 IL1b Rabbit pAb ABclonal Cat #A16288 NF-kB p65 (D14E12) Rabbit mAb Cell Signaling Technology Cat #8242S p38 MAPK (D13E1) Rabbit mAb Cell Signaling Technology Cat #8690S Phospho-p38 MAPK (Thr180/Tyr182) Rabbit mAb Cell Signaling Technology Cat #4511S Stat3 (D3Z2G) Rabbit mAb Cell Signaling Technology Cat #12640 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb Cell Signaling Technology Cat #9145 Stat1 (D1K9Y) Rabbit mAb Cell Signaling Technology Cat #14994 Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb Cell Signaling Technology Cat #8826S Erk1/2 Rabbit mAb Cell Signaling Technology Cat #4695S Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mAb Cell Signaling Technology Cat #4370S Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat #3033S F4/80 Monoclonal Antibody (BM8) eBioscience Cat #14-4801-82 Phospho-NF-kB p65 (Ser536) Antibody Affinity Cat #AF2006 IL-10 neutralizing antibody eBioscience Cat #16-7102-81 Bacterial and virus strains Mtb strain of H37Rv American Type Culture Collection NO.7294 Chemicals, peptides, and recombinant proteins SB203580 Selleckchem Cat #S1076 JSH23 Selleckchem Cat #S7351 Lactate Selleckchem Cat #E4473 Experimental models: cell lines Raw 264.7 American Type Culture Collection, USA N/A

Techniques: Infection, Expressing, Activation Assay